The objective of this study is to specifically evaluate in vitro, the antifungal properties by assaying the crude extracts obtained from some edible Malaysian fern species. Methanolic extracts of leaves, stems and roots of the local ferns Stenochlaena palustris, Diplazium esculentum, Nephrolepis biserrata and Acrostichum aureum were screened against Aspergillus niger, Rhizopus stolonifer and Candida albicans. Tests using disc diffusion method showed a broad spectrum of antifungal activity especially for Diplazium esculentum leaves. The Minimum Inhibitory Concentration (MIC) and Minimum Fungal Concentration (MFC) were also obtained against the three pathogenic fungal strains, and compared to commercial antibiotic micanazole nitrate. Values of MIC of Diplazium leaves for Aspergillus niger, Rhizopus stolonifer and Candida albicans are 100mg/mL, 50mg/mL and 50mg/mL respectively, whereas values of MFC were 200mg/mL, 100mg/mL and 100mg/mL respectively. The study shows that the fern extracts are favourable antifungal agents with potential applications in public heath against diseases. More scientific information available about the antipathogenic properties of these fern extracts is highly demanded and recommended. With a greater popularization of these aspects of properties, there will be hopefully a noticeable change in our traditional knowledge and habits regarding traditional folk medicines and fern consumption.
|Keywords:||Antifungal Activity, Stenochlaena Palustris, Diplazium Esculentum, Nephrolepis Biserrata, Acrostichum Aureum, Minimum Inhibitory Concentration (MIC), Minimum Fungal Concentration (MFC)|
University Lecturer, School of Distance Education, Universiti Sains Malaysia, Minden, Penang, Malaysia
Biology Programme, Sunway University College, Petaling, Jaya, Malaysia
Lecturer, Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Penang, Penang, Malaysia
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